N6′, N6′′′, and O4′ Modifications to Neomycin Affect Ribosomal Selectivity without Compromising Antibacterial Activity
نویسندگان
چکیده
منابع مشابه
Chloroplast ribosome structure. Electron microscopy of ribosomal subunits and localization of N6,N6-dimethyladenosine by immunoelectronmicroscopy.
Ribosomal subunits from the chloroplasts of Alaskan peas have been studied by immunoelectronmicroscopy. Electron micrographs of negatively stained small and large ribosomal subunits show particles of similar size and in the same characteristic projections described for the ribosomal subunits of Escherichia coli (Lake, J. A. (1976) J. Mol. Biol. 105, 131-159), although minor structural differenc...
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The adenosine derivative N6,N6-dimethyladenosine (DMA) shows dual fluorescence in solvents of different polarity. In addition to the “normal” fluorescence at 330 nm, another band is observed at 500 nm. The long wavelength emission dominates in aprotic solvents but is dynamically quenched by protic solvents. Steady-state and lifetime measurements show that the emissions originate from two excite...
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RNA modifications are ubiquitous in biology, with over 100 distinct modifications. While the vast majority were identified and characterized on abundant noncoding RNA such as tRNA and rRNA, the advent of sensitive sequencing-based approaches has led to the discovery of extensive and regulated modification of eukaryotic messenger RNAs as well. The two most abundant mRNA modifications-pseudouridi...
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Recently, we demonstrated that the natural cytokinin nucleosides N6-isopentenyladenosine (iPR) and N6-benzyladenosine (BAPR) exert a potent and selective antiviral effect on the replication of human enterovirus 71. In order to further characterize the antiviral profile of this class of compounds, we generated a series of fluorinated derivatives of BAPR and evaluated their activity on the replic...
متن کاملPMR study of N6, N6-dimethyladenosine conformations under high pressure.
High hydrostatic pressure affects in various ways the reactivity and the stability of biogenic macromolecules [ 11. Pressures of several hundred bars reduce or increase enzymic activity [2-41. Proteins are usually irreversibly denaturated by pressures in excess of 1 kbar. On the other hand, calf thymus DNA is stabilized by increased pressure [5]. In general, an increase in pressure shifts an eq...
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ژورنال
عنوان ژورنال: ACS Infectious Diseases
سال: 2017
ISSN: 2373-8227,2373-8227
DOI: 10.1021/acsinfecdis.6b00214